Distribution of individual %DFI values. Green bars: 18 samples from healthy sperm donors; Green bars: 39 semen samples from patients with testicular cancer after unilateral orchiectomy before further treatment. Gray bars: (Fossa et al. 1997.)
In general, men with good DNA integrity before chemotherapy have better recovery of DNA integrity after chemotherapy compared to poor DNA integrity as a starting point. Patients with diseases of the testis, Hodgkin’s, non-Hodgkin’s lymphoma and other neoplasms (abnormal growth = tumor) were evaluated using the SCSA test. The results showed that the group of cancer patients showed significantly higher levels of DNA damage than the donors who were proven to be fertile (Kobayashi et al. 2001).
In another study, the sperm of six men with acute lymphoblastic leukemia were analyzed for DNA fragmentation 10 to 52 months after completing therapy. All test subjects showed relatively low DNA fragmentation. After treatment, one patient fathered a normal child and a second child with multiple congenital anomalies, followed by a second normal child (Evenson et al. 1984).
Testicular cancer patients were evaluated by SCSA after unilateral orchiectomy (removal of a testicle) but before treatment. Twenty-three of the 39 patients showed abnormal DNA fragmentation during pretreatment.
After treatment with radiotherapy or chemotherapy (19 patients), recovery of spermatogenesis was observed in 4 out of 5 patients, with recovery occurring predominantly in those patients with normal DNA fragmentation during pretreatment. (Fossa et al. 1997).
In general, men with good DNA integrity prior to chemotherapy have better DNA integrity recovery after chemotherapy than those with poor DNA integrity prior to chemotherapy. Patients with testicular, Hodgkin’s disease, non-Hodgkin’s disease and other neoplasm’s (abnormal growth, i.e. tumor) were evaluated with SCSA analysis. The results showed the cancer patients as a group had significantly higher DNA damage when compared to proven fertile donors (Kobayashi et al. 2001).
In another study, semen from six men with acute lymphocytic leukemia was analyzed for DNA fragmentation 10 to 52 months after completion of therapy. All men had DNA fragmentation consistent with a normal fertile reproductive status. After treatment one patient fathered a normal child and a second fathered a child with multiple congenital malformations followed by a second normal child (Evenson et al. 1984).
Patients with testicular cancer were evaluated by SCSA after unilateral orchiectomy (removal of one testis) but before treatment. Twenty-three of the thirty-nine patients displayed abnormal DNA fragmentation during pre-treatment.