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Effect of vaginal lubricating gels on sperm motility and chromatin integrity: a prospective comparative study.
Agarwal A, Deepinder F, Cocuzza M, Short RA, Evenson DP.
Reproductive Research Center, Glickman Urological Institute and Department of Obstetrics-Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA. agarwaa@ccf.org
TARGET: To evaluate the effect of Lubricant Pre-Seed, FemGlide, Astroglide and Replens on human sperm motility and chromatin integrity. DESIGN: Prospective, comparative in vitro study. LOCATION: Andrology laboratory in a tertiary care hospital. PATIENT(S): 13 sperm donors with documented normozoospermia. INTERVENTION(S): 1) Incubation of 13 seed samples each in human tubal fluid media (HTF) spiked with 10% each of Pre-Seed, FemGlide, Astroglide and Replens lubricating gel. After an incubation period of 30 minutes, the progressive sperm motility was assessed under the light microscope. 2) Samples from 12 patients were placed in positive control (HTF), negative control (10% KY Jelly lube), and 10% vol/vol each of Pre-Seed and FemGlide lube. After 4 hours of culture, the DFI of the spermatozoa was determined using the acridine orange-based sperm chromatin structure assay (SCSA). MAIN MEASUREMENT PARAMETERS: Sperm motility and percentage DNA fragmentation index. RESULTS: 1) Percent motility was not significantly different between HTF controls and pre-seed, whereas FemGlide, Replens and Astroglide lubricant showed a significant decrease in motility. 2) There was no significant difference in percent DNA fragmentation index between the HTF controls and Pre-Seed, but there was a significant deterioration in sperm chromatin quality with FemGlide and KY Jelly. CONCLUSIONS:
Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study.
Agarwal A, Deepinder F, Cocuzza M, Short RA, Evenson DP.
Reproductive Research Center, Glickman Urological Institute and Department of Obstetrics-Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA. agarwaa@ccf.org
OBJECTIVE: To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity. DESIGN: Prospective, comparative, in vitro study. SETTING: Andrology laboratory at tertiary care hospital. PATIENT(S): Thirteen normozoospermic donors. INTERVENTION(S): Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre-Seed, FemGlide, Astroglide, and Replens lubricants. After 30 minutes, progressive sperm motility was assessed by light microscopy. Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre-Seed and FemGlide lubricants. After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay. MAIN OUTCOME MEASURE(S): Sperm motility and percent DNA fragmentation index. RESULTS: Percent motility did not differ significantly between HTF controls and Pre-Seed, whereas FemGlide, Replens, and Astroglide lubricants demonstrated a significant decrease in motility. There was no significant difference in percent DNA fragmentation index between the HTF controls and Pre-Seed, but a significant decline in sperm chromatin quality occurred with FemGlide and K-Y Jelly. CONCLUSION: Pre-Seed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples.
PMID: 17509584 [PubMed – indexed for MEDLINE]